Artifact-free 3D Reconstruction for Optical Projection Tomography

Optical Projection Tomography (OPT) is a recently developed implementation of computed tomography (CT) techniques at optical frequencies. A series of 2D optical projections through a sample are generated at varying orientations, from which the 3D structure of the sample can be computationally reconstructed. OPT is especially suitable for samples from about 0.5 mm to 15 mm in size, which fills an important “imaging gap” between techniques such as confocal microscopy (useful for smaller samples) and large-sample methods such as fluorescence molecular tomography (FMT), x-ray CT or microscopic magnetic resonance imaging (μΜRI). OPT can function in both fluorescence and transmission modes.